Results: Analysis of Bacterial Proteins Using Mass Spectrometry
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Conclusion
Transcript
The protocol demonstrates a rapid method for identification of bacterial proteins using antibiotic induction and mass spectrometry. The main advantage of the technique is it's speed and simplicity. Sample preparation is uncomplicated.
The te
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Here we present a protocol for the rapid identification of proteins produced by genomically sequenced pathogenic bacteria using MALDI-TOF-TOF tandem mass spectrometry and top-down proteomic analysis with software developed in-house. Metastable protein ions fragment because of the aspartic acid effect and this specificity is exploited for protein identification.