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21.8K Views
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06:38 min
August 2nd, 2021
DOI :
10.3791/62652-v
Chapters
0:05
Introduction
0:56
Culturing and Maintenance of THP‐1 Monocyte-like Cells
2:59
Seeding of THP‐1 cells and Differentiation into M0 Macrophages
4:01
Polarization of M0 Macrophages into M2‐like Macrophages
4:39
Detaching and Harvesting Macrophages for Flow Cytometry
5:14
Results: Macrophage Differentiation and Polarization into an M2‐Like Phenotype
5:56
Conclusion
Transcript
抗炎肿瘤相关的巨噬细胞与癌症的进展和预后差有关。此协议是一个指南,在 14 天内将 THP-1 单细胞状细胞分化并分化为 M2 样巨噬细胞。目前没有既定的抗炎THP-1极化模型。
此协议使用足够的休息时间和长时间的极化过程来创建独特的 M2 样巨噬体亚型。肿瘤发育,例如结肠癌和组织重塑,由抗炎巨噬细胞控制。在研究其功能的研究中,标准化地创建独特的 M2 样亚型可确保可重复的发现。
首先设置一个定时器 150 秒。将含有THP-1细胞线的冷冻小瓶从液氮中取出,在37摄
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Summary
M2样肿瘤相关巨噬细胞(TAM)与肿瘤进展和癌症预后不佳有关。本协议作为在 14 天内可重复区分和分化 THP-1 单细胞状细胞成 M2 样巨噬细胞的详细指南。该模型是研究TAM在肿瘤微环境内的抗炎作用的基础。
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