Results: Conformational Characterization of Proteins with Top-Down Mass Spectrometry
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Conclusion
Transcript
This method allows the separation of coexisting protein species, including different conformers or variants and online characterization of their higher order structure differences to be completed in a single CEMS measurement. It utilizes the elect
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Presented here is a protocol for a capillary electrophoresis-based hydrogen/deuterium exchange (HDX) approach coupled with top-down mass spectrometry. This approach characterizes the difference in higher-order structures between different protein species, including proteins in different states and different proteoforms, by conducting concurrent differential HDX and electrophoretic separation.