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Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

5.5K Views

06:06 min

July 22nd, 2021

July 22nd, 2021

5,484 Views

0:05

Introduction

0:56

Sample Processing

1:54

Hard Tissue Homogenization

3:05

Soft Tissue Homogenization

4:20

Results: Tissue Identification and RNA Quality Assessment

5:27

Conclusion

Transcript

This protocol encourages the use of primary human tissues as a clinically relevant model for osteoarthritis research. Performing gene expression and histological analyses in these tissues may provide novel mechanistic insights. This technique stan

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Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis.

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