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DOI :
10.3791/62733-v
August 19th, 2021
Chapters
0:05
Introduction
0:28
Measuring Macropinosome pH
2:06
In Situ Calibration of Macropinosome pH
3:04
Measuring Oxidative Events within Macropinosomes
4:22
Measuring Protein Digestion within Macropinosomes
5:30
Results: Measuring the pH, Oxidative Events, and Protein Degradation in Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
6:59
Conclusion
生細胞の個々のマクロピノソームにおけるpH、酸化事象、タンパク質消化を測定するためのプロトコルについて述べています。二重蛍光系比測定顕微鏡と、人口ベースの技術よりも利点に重点が置かれています。
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