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DOI :
10.3791/62733-v
August 19th, 2021
Chapters
0:05
Introduction
0:28
Measuring Macropinosome pH
2:06
In Situ Calibration of Macropinosome pH
3:04
Measuring Oxidative Events within Macropinosomes
4:22
Measuring Protein Digestion within Macropinosomes
5:30
Results: Measuring the pH, Oxidative Events, and Protein Degradation in Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
6:59
Conclusion
우리는 살아있는 세포에 있는 개별 적인 macropinosomes에 있는 pH, 산화 사건 및 단백질 소화를 측정하기 위한 프로토콜을 기술합니다. 이중 불소 경 미세 검사법과 인구 기반 기술에 비해 제공하는 이점에 중점을 둡니까.
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