Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

0:22

Prepare the Manual Plunging Environment

0:42

Prepare the Cryogen Dewar and Manual Plunger

2:04

Prepare the Cryogen

4:36

Prepare Plunging Materials and Accessories

5:13

Prepare CryoEM Specimens by Plunge Freezing

7:22

Representative Results

7:50

Conclusion