Quantitative Real-time PCR and Fluorescence Microscopy
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Results: Light-activated Gene Expression Assessment in Zebrafish Embryo
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Conclusion
Transcript
This protocol is significant because it is an optogenetic, or light-activated method, for inducing gene expression, in an intact organism. In our case, a zebrafish embryo. The advantage of this technique, is that it uses light, as an inducing agen
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Optogenetics is a powerful tool with wide-ranging applications. This protocol demonstrates how to achieve light-inducible gene expression in zebrafish embryos using the blue light-responsive TAEL/C120 system.