Determination of Mycobacterium tuberculosis (Mtb) Uptake by Macrophages: Multiplicity of Infection (MOI)
2:30
Acid Fast Bacteria Staining
4:14
Macrophage Infection
5:09
Sample Collection
6:10
Inoculating and Loading Instrument Culture Bottles
7:22
Results:Mycobacterium tuberculosis Growth Quantification and Therapy Evaluation Using Automated Culture System
8:54
Conclusion
Transcript
Het grote voordeel van dit protocol is dat het minder arbeidsintensief en tijdrovend is dan de traditionele methode om kolonievormende eenheden te tellen om de intracellulaire groei van Mycobacterium tuberculosis te schatten. Deze techniek maakt h
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Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.