Determination of Mycobacterium tuberculosis (Mtb) Uptake by Macrophages: Multiplicity of Infection (MOI)
2:30
Acid Fast Bacteria Staining
4:14
Macrophage Infection
5:09
Sample Collection
6:10
Inoculating and Loading Instrument Culture Bottles
7:22
Results:Mycobacterium tuberculosis Growth Quantification and Therapy Evaluation Using Automated Culture System
8:54
Conclusion
Transcript
Der Hauptvorteil dieses Protokolls besteht darin, dass es weniger arbeitsintensiv und zeitaufwendig ist als die traditionelle Methode zur Zählung koloniebildender Einheiten, um das intrazelluläre Wachstum von Mycobacterium tuberculosis abzuschätze
Sign in or start your free trial to access this content
Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.