Determination of Mycobacterium tuberculosis (Mtb) Uptake by Macrophages: Multiplicity of Infection (MOI)
2:30
Acid Fast Bacteria Staining
4:14
Macrophage Infection
5:09
Sample Collection
6:10
Inoculating and Loading Instrument Culture Bottles
7:22
Results:Mycobacterium tuberculosis Growth Quantification and Therapy Evaluation Using Automated Culture System
8:54
Conclusion
Transcript
Il vantaggio principale di questo protocollo è che è meno laborioso e richiede tempo rispetto al metodo tradizionale di conteggio delle unità formanti colonie per stimare la crescita intracellulare di Mycobacterium tuberculosis. Questa tecnica ren
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Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.