Determination of Mycobacterium tuberculosis (Mtb) Uptake by Macrophages: Multiplicity of Infection (MOI)
2:30
Acid Fast Bacteria Staining
4:14
Macrophage Infection
5:09
Sample Collection
6:10
Inoculating and Loading Instrument Culture Bottles
7:22
Results:Mycobacterium tuberculosis Growth Quantification and Therapy Evaluation Using Automated Culture System
8:54
Conclusion
Transcript
Bu protokolün temel avantajı, Mycobacterium tuberculosis'in hücre içi büyümesini tahmin etmek için koloni oluşturan birimleri saymanın geleneksel yönteminden daha az emek yoğun ve zaman alıcı olmasıdır. Bu teknik, araştırmacıların FDA onaylı ilaçl
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Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.