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JoVE Journal

Biochemistry

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Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

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08:55 min

July 19th, 2021

July 19th, 2021

2,703 Views

0:04

Introduction

0:33

Yeast Cells Growth

1:37

Isolation of Highly Purified Mitochondria

2:03

Sub-Mitochondrial Fractionation: Hypotonic Swelling Using Proteinase K

4:01

Sub-Mitochondrial Fractionation: Sonication and Carbonate Extraction

6:31

Results: Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

8:14

Conclusion

Transcript

This protocol is designed to determine the submitochondrial localization of yeast mitochondrial proteins, which is considered as a fundamental step during mitochondrial protein function elucidation. The protocol is suitable for our yeast strains m

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Despite recent advances, many yeast mitochondrial proteins still remain with their functions completely unknown. This protocol provides a simple and reliable method to determine the submitochondrial localization of proteins, which has been fundamental for the elucidation of their molecular functions.

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