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10.9K Views
•
07:58 min
October 7th, 2021
DOI :
10.3791/62876-v
Chapters
0:04
Introduction
0:50
Perfusion
2:57
Cryosectioning
4:06
Free-Floating Wisteria Florbunda Agglutinin Staining and Anti- Arginine Vasopressin Immunostaining
5:13
Mounting
6:00
Imaging
6:42
Results: Double Immunofluorescent Staining of Free-Floating Mouse Brain Sections
7:21
Conclusion
Transcript
小鼠大脑的免疫组织化学染色是神经科学研究中常用的技术。然而,脑组织学结果的质量,可靠性和可重复性可能因不同的研究人员和实验室而异。我们提出了一种既定的小鼠大脑组织学研究方法,该方法已被证明是可重复的,可靠的和有效的。
帮助我演示该程序的将是来自我实验室的助理教授Chunmei Wang博士 在确认在8至16周龄的C57黑色/ 6J小鼠中成功麻醉后,将鼠标固定在泡沫板上,并沿着胸部和腹部的中线做一个纵向浅表切口,然后将皮肤移到一边以暴露胸部和腹部的肌肉壁。接下来,在肌肉层做一个切
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Summary
该协议描述了一种用于小鼠脑组织学研究的有效且可重复的方法,包括灌注,脑切片,自由漂浮的免疫染色,组织安装和成像。
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