JoVE Journal
Biochemistry
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11:03 min
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September 9th, 2021
DOI :
September 9th, 2021
•Chapters
0:04
Introduction
0:56
Liver Perfusion and Dissociation
4:00
Fatty Acid β-oxidation Assay
8:59
Results: The Measurement of Fatty Acid β-Oxidation in a Mouse Hepatocytes Suspension Incubated with 14C-Labeled Palmitic Acid
10:24
Conclusion
Transcript
This protocol was developed to provide a robust method for the measurement of total mitochondrial and peroxisomal fatty acid beta-oxidation in primary mouse hepatocytes. The use of intact cells maintains organelle integrity and the regulatory mech
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Summary
Fatty acid β-oxidation is an essential metabolic pathway responsible for generating energy in many different cell types, including hepatocytes. Here, we describe a method to measure fatty acid β-oxidation in freshly isolated primary hepatocytes using 14C-labeled palmitic acid.
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