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3.6K Views
•
08:45 min
August 4th, 2021
DOI :
10.3791/62914-v
Chapters
0:04
Introduction
1:21
Hydrating Sensor Cartridge and Preparation of the Assay Medium
1:55
Coating Mesh Inserts of Islet Capture Microplate
2:35
Retinal Dissection and Retinal Punch Preparation
4:21
Loading the Sensor Cartridge Injection Ports and Calibration
5:09
Loading the Islet Capture Plate and Running Assay
6:31
Results: Mitochondrial Stress and Glycolytic Rate Assays
7:51
Conclusion
Transcript
该协议提供两种主要生物能量途径的实时分析:线粒体呼吸和新鲜解剖的离体小鼠视网膜组织中的糖酵解。传统的海马分析集中在培养的单层细胞上,而该协议允许研究人员研究新鲜解剖的组织(特别是视网膜)中的线粒体活性。该技术用于研究遗传性视网膜变性小鼠模型的视网膜中的线粒体活性和糖酵解标志。
它是视网膜研究的有用工具,也有潜力应用于其他类型的组织,以开发潜在的治疗方法。该协议最困难的部分是从新鲜解剖的小鼠视网膜中获得高质量的打孔盘,因此建议将执行此技术的个人具有良好的视网膜解剖技能。在实验前一
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Summary
这里描述的是使用商用生物分析仪在 离体 视网膜组织样品中进行线粒体应激测定和糖酵解速率测定的详细方案。
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