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5.7K Views
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07:14 min
February 25th, 2022
DOI :
10.3791/62932-v
Chapters
0:04
Introduction
1:36
Preparation of Light Emitting Diode (LED) for Blue Light Illumination
2:13
Imaging of Zebrafish Larvae Expressing Optogenetic TAR DNA-binding Protein 43 (TDP-43)
3:58
Light Stimulation of Optogenetic TDP-43h-Expressing Fish by Field Illumination of a Blue LED Light
4:35
Results: Characterization of TDP-43 Phase Transition in Spinal Motor Neurons of Zebrafish Larvae
6:34
Conclusion
Transcript
因此,该协议使我们能够诱导TDP-43的病理相变,并解决它们在体内脊髓运动神经元中的细胞后果,这与理解为什么运动神经元在ALS中退化有关。由于斑马鱼幼虫的高透明度,脊髓中的运动神经元是直接可见的。因此,人们可以想象TDP-43在单个脊柱运动神经元中经历相变的动态行为。
该方法提供了ALS的新型动物模型。我们认为,在该ALS模型中,任何可以防止光诱导的TDP-43相变和聚集的方法都可能是ALS治疗的潜在候选者。可以表达具有内在无序区域的其他ALS相关蛋白,以探索与异常相变相关的神
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Summary
我们描述了一种以斑马鱼为模型的模式,通过光在脊髓运动神经元中诱导TAR DNA结合蛋白43(TDP-43)的相变。
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