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Live-imaging of Mitochondrial System in Cultured Astrocytes

DOI :

10.3791/62957-v

6:20 min

November 16th, 2021

November 16th, 2021

3,215 Views

1Department of Psychiatry, Center for Psychiatric Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne

This article describes the method for mitochondrial time-lapse imaging of astrocyte cultures equipped with MitoTimer biosensor and the resulting multiparametric analysis of mitochondrial dynamics, mobility, morphology, biogenesis, redox state, and turnover.

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Live imaging

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