JoVE Logo
Faculty Resource Center

Sign In

Live-imaging of Mitochondrial System in Cultured Astrocytes

DOI :

10.3791/62957-v

6:20 min

November 16th, 2021

November 16th, 2021

3,398 Views

1Department of Psychiatry, Center for Psychiatric Neurosciences, Lausanne University Hospital (CHUV) and University of Lausanne

This article describes the method for mitochondrial time-lapse imaging of astrocyte cultures equipped with MitoTimer biosensor and the resulting multiparametric analysis of mitochondrial dynamics, mobility, morphology, biogenesis, redox state, and turnover.

Tags

Live imaging

-- Views

Related Videos

article

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons

article

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

article

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations

article

Live Imaging of Dorsal Root Axons after Rhizotomy

article

High-resolution Live Imaging of Cell Behavior in the Developing Neuroepithelium

article

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

article

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

article

Live Imaging of Drosophila Larval Neuroblasts

article

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes

article

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved