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JoVE Journal

Neuroscience

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Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability

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07:23 min

August 6th, 2021

August 6th, 2021

2,481 Views

0:05

Introduction

0:45

Laser Setup

2:05

Mouse Tail Vein Injection of Rose Bengal

2:54

Occlusion of Major Veins

4:07

Establishing the Number of Veins Occluded at Day 0 and Aftercare

4:40

Results: Analysis of Types and Timelines of Photo-Occlusion, Baseline and Experimental Laser Output Measurement, Ophthalmic Pathologies, and Quantification of OCT Images

6:37

Conclusion

Transcript

The protocol provides a model to investigate mechanisms of vascular injury and subsequent development of edema in a non-invasive manner. The main advantage of this technique is that you can follow the injury live over time without surgical interve

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Here, we describe an optimized protocol for retinal vein occlusion using rose bengal and a laser-guided retinal imaging microscope system with recommendations to maximize its reproducibility in genetically modified strains.

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