September 10th, 2021
•This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.
Related Videos
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans Video (Video) | JoVE
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification Video (Video) | JoVE
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy Video (Video) | JoVE
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins Video (Video) | JoVE
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Video (Video) | JoVE
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation Video (Video) | JoVE
Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System Video (Video) | JoVE
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells Video (Video) | JoVE
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells Video (Video) | JoVE
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens Video (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved