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Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

DOI :

10.3791/63008-v

September 10th, 2021

September 10th, 2021

2,967 Views

1Department of Entomology and Agrilife Research, Texas A&M University

This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.

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