Transferring UDG Reaction Products to a Matrix Chip
5:33
Setting up the Assay Parameters on the Mass Spectrometer
7:19
Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data
9:03
Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis
11:01
Conclusion
Transcript
DNA repair mechanisms are very important for maintaining genome integrity in all living organisms. Uracil-DNA glycosylase is a crucial DNA repair protein in the basic tissue repair pathway. Using MALDI-TOF mass spectrometry, we can directly detect
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A non-labeled, non-radio-isotopic method to assay uracil-DNA glycosylase activity was developed using MALDI-TOF mass spectrometry for direct apurinic/apyrimidinic site-containing product analysis. The assay proved to be quite simple, specific, rapid, and easy to use for DNA glycosylase measurement.