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1.2K Views
•
14:56 min
April 21st, 2023
DOI :
10.3791/63188-v
Chapters
0:03
Introduction
1:17
Krebs-Henseleit Buffer Preparation
1:45
Perfusion System Preparation
2:29
Calibration and Preparation of the NMR Spectrometer for Acquisition
3:22
Animal Preparation, Surgical Procedure, and Perfusion of the Heart in the NMR Tube
9:23
Acquiring Data for Cardiac Energetics and pH
10:09
DNP Spin Polarization and Dissolution
11:25
Hyperpolarized 13C Spectroscopy
12:07
Representative Results
14:40
Conclusion
Transcript
Hi.In 本视频,您将学习如何对灌注的小鼠心脏进行超极化MR研究。David Shaul是实验室的MD / PhD学生,将指导您完成各种技术和程序。今天,我们学习了一种研究超极化碳-13丙酮酸代谢的方案,并结合了31P NMR波谱。
丙酮酸代谢处于有氧和厌氧代谢的十字路口。因此,它对于研究心脏的各种状况具有重要价值。所以,让我们开始吧。
分离小鼠心脏并灌注Krebs-Hensleit缓冲液,在10毫米NMR管内。然后将心脏插入核磁共振波谱仪。在那里,通过观察三磷酸
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Summary
我们描述了一种实验装置,用于在连续灌注模式下向分离的灌注小鼠心脏施用超极化 13种C标记代谢物。专用的 13C-NMR采集方法能够实时定量代谢酶活性,多参数 31P-NMR分析可以测定组织ATP含量和pH值。
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