Undersøgelse af hjertemetabolisme i det isolerede perfunderede musehjerte med hyperpolariseret [1-13 C] pyruvat og ¹³C / ³¹P NMR spektroskopi

1:17

Krebs-Henseleit Buffer Preparation

1:45

Perfusion System Preparation

2:29

Calibration and Preparation of the NMR Spectrometer for Acquisition

3:22

Animal Preparation, Surgical Procedure, and Perfusion of the Heart in the NMR Tube

9:23

Acquiring Data for Cardiac Energetics and pH

10:09

DNP Spin Polarization and Dissolution

11:25

Hyperpolarized ¹³C Spectroscopy

12:07

Representative Results

14:40

Conclusion