Генерация нулевых мутантов для выяснения роли бактериальных гликозилтрансфераз в бактериальной подвижности

1:01

Construction of pDM4 Recombinant Plasmid with PCR In-Frame Deletion Products

3:10

Preparation of Electrocompetent E. coli and Electroporation of pDM4 Recombinant Plasmid

4:52

Transfer of In-Frame Deletion to the Aeromonas Strain

7:44

Motility Assays in Liquid and Semi-Solid Media

8:34

Isolation and Purification of Flagella

10:23

Results: Confirmation of Null Mutants and Phenotypic Analysis of Polar Flagella

11:47

Conclusion