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DOI :
10.3791/63266-v
•
7:48 min
March 1st, 2022
Chapters
0:05
Introduction
0:45
Preparation of Heat-Killed Bacteria Expressing Double-Stranded RNA (dsRNA)
2:09
Feeding Mosquitoes with Heat-Killed Bacteria Expressing dsRNA
2:47
Assay Target Gene Expression Levels
3:59
Phenotypic Evaluation: Successful Blood-Feeding
4:35
Phenotypic Evaluation: Salivary Gland Morphology and Down-Regulation of Relevant Proteins
5:30
Results: The Effects of fkh Knockdown in Adult Anopheles gambiae Salivary Glands
7:17
Conclusion
細菌によって産生されるdsRNAの経口投与は、 カエノラブディティス・エレガンス において日常的に使用されているRNA干渉(RNAi)の送達方法であり、ここで成虫の蚊に適用することに成功した。我々の方法は、注射を使用せずに堅牢な逆遺伝学研究および伝達遮断ベクター研究を可能にする。
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