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Encapsulamento rápido do Citoesqueleto Reconstituído Dentro de Vesículos Unilamellar Gigantes

DOI :

10.3791/63332-v

November 10th, 2021

November 10th, 2021

3,690 Views

1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor

Este artigo introduz um método simples para a produção rápida de vesículas unilamellar gigantes com proteínas citoesqueletal encapsuladas. O método se mostra útil para a reconstituição de estruturas citoesqueletal em interações de confinamento e citoesqueleto-membrana.

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Bioengenharia

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