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Snabb inkapsling av rekonstituerad cytoskelett inuti jätte unilamellarblåsor

DOI :

10.3791/63332-v

November 10th, 2021

November 10th, 2021

3,690 Views

1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor

Denna artikel introducerar en enkel metod för snabb produktion av gigantiska unilamellarblåsor med inkapslade cytoskelettproteiner. Metoden visar sig vara användbar för bottom-up rekonstitution av cytoskelettstrukturer i inneslutning och cytoskelett-membraninteraktioner.

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Bioteknik

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