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2.7K Views
•
07:32 min
December 28th, 2021
DOI :
10.3791/63355-v
Chapters
0:05
Introduction
1:00
Zebrafish Maintenance and Pre-ICV Microinjection Preparations
2:19
Anaesthetisation and ICV Injection of Zebrafish
4:10
Locomotor Assessment
5:22
Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
6:44
Conclusion
Transcript
该方案在显示斑马鱼运动障碍和随后在腹侧间脑室注射神经毒素 6-羟基多巴胺后恢复方面具有重要意义。该技术展示了功能效应的变化,这对应于使用非常简单的设置的6-OHDA病变的成年斑马鱼腹侧间脑中多巴胺能神经元的特定消融。未来对神经再生机制的研究,以及调节该过程的内在和外在因素,可能为针对帕金森病的细胞替代治疗策略提供重要的见解。
将鱼保持在充气蒸馏矿化水箱中,每升商业海盐一克,控制温度为28,加或减一摄氏度。每45升水箱最多容纳25条鱼,并将它们暴露在14小时的光线和10小时的黑暗
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Summary
本方案描述了在腹侧间脑(Dn)处用神经毒性6-羟基多巴胺(6-OHDA)向成年斑马鱼注射脑室(ICV)以及通过使用开放水箱测试评估损伤和随后恢复的游泳行为,并伴有使用视频跟踪软件进行分析。
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