Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
1.7K Views
•
08:01 min
April 21st, 2022
DOI :
10.3791/63374-v
Chapters
0:05
Introduction
0:45
Digestion of Tumor Tissue
3:16
Generation of Primary Tumor Cell Line
4:47
Characterization and Banking of Established Primary Tumor Cell Line
6:13
Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
7:12
Conclusion
Transcript
该协议为肿瘤细胞扩增提供了基础,反过来,允许对肿瘤细胞功能和基因组状态进行深入研究。此外,它还提供了一个体外模型系统来评估肿瘤对免疫反应的影响。该技术的主要优点是它提供了一种基于方案,用于从患者组织建立间皮肿瘤谱系。
目前,此模型系统没有标准化协议。该技术最困难的方面是在早期传代培养中存在成纤维细胞污染。对成纤维细胞与肿瘤细胞形态有深入的了解对于确定成纤维细胞饥饿尝试是否有效至关重要。
首先将10毫升肿瘤消化酶混合物转移到解离管中。使用无菌手术刀将肿瘤组织转移到无菌
Sign in or start your free trial to access this content
Summary
本方法论文的目的是证明一种可靠且可重复的方法,用于从手术切除的胸膜间皮瘤中富集,生成和扩增原发性肿瘤细胞系。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved