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2.7K Views
•
07:41 min
June 8th, 2022
DOI :
10.3791/63379-v
Chapters
0:04
Introduction
0:57
Hydrating the Cartridge Sensors
1:45
Preparing the Tissue Plate
2:24
Acute Striatal Slice Preparation and Excised Slice Placement
4:11
Loading of Cartridges with Desired Compounds
5:17
Calibration and Performing the Assay
6:07
Results: Measuring the Oxygen Consumption Rates in Acute Striatal Slices from Adult Mice
7:11
Conclusion
Transcript
我们开发了一种使用海马XF分析仪直接测量耗氧率的新方法。但线粒体功能的常见代理在成年小鼠的急性切片中。这种方法通过解剖学定义的大脑结构的穿刺能量学来测量细胞。
使用急性切片更接近地模仿生理细胞环境,这是无法实现分离线粒体或培养细胞的方式。这种方法将引起在帕金森病和亨廷顿舞蹈症领域工作的研究人员的广泛兴趣。首先,打开细胞外通量测定试剂盒,取出电极盒和实用板。
然后将传感器盒放在一边,不要触摸传感器。接下来,将600微升的校准溶液添加到实用板的每壁上。将传感器盒放在实用
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Summary
耗氧率(OCR)是线粒体功能的常见代理,可用于研究不同的疾病模型。我们开发了一种使用Seahorse XF分析仪的新方法,直接测量成年小鼠急性纹状体切片中的OCR,该方法比其他方法更具生理相关性。
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