Results: Förster Resonance Energy Transfer Mapping of the Preprotein Binding Site
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Conclusion
Transcript
We have developed a FRET mapping methodology that allows the identification and characterization of ligand binding sites, subunit orientation, conformational changes associated with ligand binding, and dynamic motions of proteins. An advantage of
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The study details the methodology of FRET mapping including the selection of labeling sites, choice of dyes, acquisition, and data analysis. This methodology is effective at determining binding sites, conformational changes, and dynamic motions in protein systems and is most useful if performed in conjunction with existing 3-D structural information.