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1.7K Views
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08:07 min
March 24th, 2023
DOI :
10.3791/63449-v
Chapters
0:04
Introduction
1:02
Microscope Settings
2:00
Microscope Preliminary Controls
3:00
Vibratome Sectioning
4:00
Transfer to the Microscope and Imaging
5:15
Processing of the Cerebellum
5:35
Results: Visualization of Tubulin-Dependent Defects in Central Myelin
6:38
Conclusion
Transcript
生物样品的二次谐波生成成像是一种光学技术,无需标签或染料即可可视化非中心对称分子及其组装。用这种方法生成的图像背景较低,因为很少有生物分子可以作为协调力。该协议描述了在taiep动物模型中微管蛋白β-4A诊断成像技术的应用。
微管蛋白病是最近描述的疾病。出于这个原因,关于细胞水平病理生理学的基本机制的信息有限。首先,打开脉冲激光器以确保它准备好以最佳和稳定的功率水平供电。
为了研究鼬岛微管,使用10%至20%的可用激光功率,在所述系统中对应于在物镜后焦平面处测量的1
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Summary
在本文中,我们提出了一种协议,通过简单,创新的二次谐波产生显微镜方法检测微管负载的少突胶质细胞在微管疾病模型中。
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