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3.2K Views
•
07:20 min
December 30th, 2021
DOI :
10.3791/63453-v
Chapters
0:04
Introduction
0:51
Isolation of Heavy Metal Resistant Microorganisms
2:50
Identification of Microbial Isolates
4:16
Heavy-Metals and Antibiotics Susceptibility
5:36
Results: Methodological Approach for Identification of Heavy Metal Tolerant Bacteria
6:52
Conclusion
Transcript
该协议描述了一种从地热泉中筛选和分离抗重金属微生物的简化方法。这种方法代表了全球对生物传感和生物修复领域应用越来越感兴趣的研究领域。其中描述的方法可以很容易地修改,以从不同的环境来源(例如水,食物,土壤或沉积物)中分离微生物。
鉴定耐药微生物的最小抑制浓度是表征新物种或菌株的快速策略。该协议易于执行,只需要手动技能和基本微生物学技术的经验。首先,将两克收集的样品接种到50微米新鲜制备的Luria-Bertani培养基中,pH值为4或7。
将此样品在采样部位的温度正负
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Summary
从地热泉中分离出抗重金属微生物是生物修复和环境监测生物系统发展的热门话题。本研究为从温泉中分离和鉴定重金属耐受细菌提供了一种方法学方法。
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