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5.4K Views
•
09:55 min
February 3rd, 2022
DOI :
10.3791/63487-v
Chapters
0:05
Introduction
0:42
Dissection of the Choroid Plexus from the Brain
2:47
Preparation of Samples for Flow Cytometry Analysis
3:52
Choroid Plexus Myeloid Cells Gating
6:46
Choroid Plexus T Cells Gating
7:44
Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
9:24
Conclusion
Transcript
该协议可能有助于解决有关脉络丛和外周免疫细胞在小鼠模型中各种情况下调节大脑中的作用的问题。该协议允许对小鼠脉络膜丛中独特的免疫细胞群进行深入的定量和定性分析。由于脉络膜丛的体积小且颜色透明,在开始时可能很难解剖脉络丛。
我们建议先在非灌注动物身上练习。首先,将C57黑色六只小鼠背侧解剖的大脑放在培养皿中,并将培养皿放在双眼环的目标下方。在使用镊子将大脑保持在适当的位置时,将另一对镊子的两端向下插入半球之间的中线。
使用镊子,将带有胼胝体和海马体的皮层从隔膜上拉开,暴
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Summary
本研究使用流式细胞术和两种不同的门控策略对分离的灌注小鼠脑脉络丛;该协议确定了填充该大脑结构的主要免疫细胞亚群。
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