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3.1K Views
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04:53 min
April 5th, 2022
DOI :
10.3791/63511-v
Chapters
0:04
Introduction
0:42
Mice Dissection and Tissue Dissociation
1:35
Debris Removal and Magnetic Separation of Microglial Cells
3:26
Results: Gating Strategy Used in Flow Cytometry Analysis Before and After MACS and Gating Strategy for Living/Dead Single Cells After MACS
4:27
Conclusion
Transcript
该方案有助于从成年小鼠大脑的微剖脱髓鞘病变中分离出小胶质细胞,以研究体内的小胶质细胞特征。这种技术节省了时间,对实验人员和设备的要求也更低。这种方法有助于从动物的大脑中分离出小胶质细胞,特别是那些处于各种疾病状态的微切片组织。
首先在立体显微镜下对胼胝体周围由中性染料标记的病变进行微剖。将解剖的组织以300倍离心30秒,以收集管底部的样品。在培养箱中预热酶混合物一和酶混合物两到37摄氏度。
然后,加入1, 950微升预热酶,一对一样品混合,并在37摄氏度的培养箱中消
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Summary
在这里,我们提出了一种方案,利用柱状磁激活细胞分选在脱髓鞘疾病的动物模型中分离和纯化原代小胶质细胞。
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