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DOI :
10.3791/63536-v
February 1st, 2022
Chapters
0:04
Introduction
1:17
Pre-Blocking of Samples to Prevent Early Digestion by MNase
1:46
Addition of Primary Antibody
3:09
Addition of pA-MNase or pA/G-MNase
4:04
Directed DNA Digestion
5:04
Sample Fractionation and DNA Extraction
7:09
Results: Protein Localization on Chromatin Using Single Cell uliCUT&RUN
8:54
Conclusion
CUT&RUN及其变体可用于确定染色质上的蛋白质占用率。该协议描述了如何使用单细胞uliCUT&RUN确定染色质上的蛋白质定位。
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