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DOI :
10.3791/63536-v
February 1st, 2022
Chapters
0:04
Introduction
1:17
Pre-Blocking of Samples to Prevent Early Digestion by MNase
1:46
Addition of Primary Antibody
3:09
Addition of pA-MNase or pA/G-MNase
4:04
Directed DNA Digestion
5:04
Sample Fractionation and DNA Extraction
7:09
Results: Protein Localization on Chromatin Using Single Cell uliCUT&RUN
8:54
Conclusion
CUT&RUN 및 그 변이체는 크로마틴에 대한 단백질 점유율을 결정하는 데 사용될 수 있다. 이 프로토콜은 단일 세포 uliCUT&RUN을 사용하여 크로마틴에 대한 단백질 국소화를 결정하는 방법을 설명합니다.
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