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2.2K Views
•
06:48 min
March 23rd, 2022
DOI :
10.3791/63552-v
Chapters
0:04
Introduction
0:53
Isolation of Leukocytes by Dextran Sedimentation of Red Blood Cells
2:57
“Mini-Extraction” of Lipids from Isolated Leukocytes
4:03
Lipid Analysis by MALDI-TOF/MS
5:00
Results: Diagnosis of Barth Syndrome Using Leukocyte CL Fingerprints and (MLCL + CLi)/CLm Ratios
6:06
Conclusion
Transcript
该方法通过MALDI-TOF质谱法生成白细胞的心磷脂指纹,并允许测量单心磷脂和心磷脂之间的比率,以快速诊断Barth综合征。该技术的主要优点是在从一毫升血液中分离白细胞后立即通过单轮质谱法检测诊断脂质物种。这些以及眼科敏感性和特异性使该测定成为一种合适的诊断测试,可以整合到临床实验室的常规工作中,以筛查Barth综合征。
首先将血液样本放在眼眶摇床上10分钟。接下来,在1.5毫升管中加入0.9毫升全血,加入0.1毫升20%葡聚糖。现在移液并轻轻分散悬浮液20次,同时避免气泡,让
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Summary
该协议显示了如何获得白细胞心磷脂的质谱“指纹”以诊断Barth综合征。单心磷脂与心磷脂比值升高的评估将 Barth 综合征患者与对照心力衰竭患者区分开来,敏感性和特异性为 100%。
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