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DOI :
10.3791/63569-v
March 16th, 2022
Chapters
0:04
Introduction
1:07
Bovine Chromaffin Cell Culture
2:52
Transfection with Electroporation
4:18
Preparation for Patch-Clamp Recording and Confocal Imaging
5:41
Patch-Clamp Recording and Confocal Imaging
9:01
Confocal Imaging Data Analysis
10:21
Results: Whole-Cell Voltage-Clamp Recordings and Visualization of Fusion Events Under Confocal Microscope
11:52
Conclusion
该协议描述了一种共聚焦成像技术,用于检测牛肾上腺嗜铬细胞中的三种融合模式。这些融合模式包括1)闭合融合(也称为亲吻和运行),涉及融合孔隙开放和闭合,2)停留融合,涉及融合孔隙开放和维持开放孔,以及3)收缩融合,涉及融合囊泡收缩。
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