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08:28 min
August 26th, 2022
DOI :
10.3791/63599-v
Chapters
0:05
Introduction
0:48
Root Processing, Clearing, and Staining for Microscopy
2:10
Root Processing for Microscopy
2:56
Microscopic Analysis of the Root Samples
3:26
Post-Microscopy Image Assemblage and Scoring of Mycorrhizal Colonization
5:05
Raw Data Analysis and Result Extraction
6:45
Results: Assessment of the Arbuscular Mycorrhizal Colonization Patterns
7:53
Conclusion
Transcript
MycoPatt方法允许深入探索根系丛枝菌根定植。菌根图指向真菌扩张,并将每个结构的真实位置呈现为特定模式。以1%的分辨率客观分析菌根定植。
每个根段都自动转换为数字矩阵,并生成一个广泛的定植参数数据库。首先,从冰箱中取出根部并将它们放在工作平台上以在室温下解冻。要进行根部清理,请将一株植物的所有根放入 30 至 50 毫升的罐子中,然后用自来水准备 10% 氢氧化钠溶液并将其倒入罐中,直到完全覆盖根部。
用力摇晃罐子一到两分钟,使清除溶液均匀分散在根部。让根在澄清
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Summary
这里的协议描述了评估丛枝菌根定植模式的方法和两个物种的根策略: Zea mays 和 Festuca rubra。MycoPatt方法的使用允许计算参数,将菌根结构转换为数字数据,以及绘制它们在根中的真实位置。
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