Canine Intestinal Organoids in a Dual-Chamber Permeable Support System

3.2K Views

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11:11 min

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March 2nd, 2022

DOI :

10.3791/63612-v

March 2nd, 2022

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Vojtech Gabriel¹, Christopher Zdyrski¹, Dipak K. Sahoo², Kimberly Dao³, Agnes Bourgois-Mochel², Todd Atherly³, Marilyn N. Martinez⁴, Donna A. Volpe⁵, Jamie Kopper², Karin Allenspach²^,³, Jonathan P. Mochel¹^,³

¹Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, ²Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, ³3D Health Solutions Inc., ⁴Office of New Animal Drug Evaluation, Center for Veterinary Medicine, Food and Drug Administration, ⁵Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, Food and Drug Administration

Transcript

Summary

Chapters in this video

0:04

Introduction

1:05

Permeable Support Seeding

5:30

TEER Value Measurement

6:51

Experimental Layout

8:09

Results: Staining, Light Microscopy, and TEM Images of Canine Intestinal Organoid Monolayer

10:36

Conclusion

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