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1.8K Views
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08:08 min
June 1st, 2022
DOI :
10.3791/63662-v
Chapters
0:04
Introduction
0:43
Cell Culture and DNA Extraction
1:55
Detection of Wolbachia Nucleic Acid and Quantitative PCR Analysis
2:53
Protein Extraction
4:26
Immunofluorescence Localization of Wolbachia Protein
6:05
Results: Detection of Intracellular Wolbachia Infection at the Nucleic acid and Protein Levels
7:21
Conclusion
Transcript
该协议有助于验证细胞中沃尔巴克氏体的存在,这是理解沃尔巴克氏体与其宿主之间相互作用的基础。该方案采用4种方法成功检测细胞内沃尔巴克氏体感染,证实并提高了沃尔巴克氏体感染细胞的检测准确率。现代病原生物学与特征重点实验室研究生肖秋秋将演示该程序。
首先使用光学显微镜以100倍放大率观察未染色的Aa23和Aa23-T细胞。将细胞培养五到七天,使每个烧瓶达到70%至80%的汇合度。使用移液管将1 mL培养基转移到微量离心管中。
在100 RCF下离心五分钟以收获细胞。除去上
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Summary
采用4种方法检测细胞内沃尔巴克氏体,相辅相成,提高了抗生素治愈白纹伊蚊来源的Aa23和Aa23-T感染的细菌感染的准确性。
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