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JoVE Journal

Biochemistry

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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

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11:38 min

April 5th, 2022

April 5th, 2022

2,259 Views

0:04

Introduction

0:59

Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition

2:44

Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand

3:56

Isothermal Titration Calorimetry

9:00

Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins

10:48

Conclusion

Transcript

This method will allow the researcher to probe hard to study systems, like peptides and first row transition metal ions, both of which are generally challenging using more standard applications. The main advantage here is using orthogonal techniqu

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This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

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