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5.0K Views
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14:10 min
July 29th, 2022
DOI :
10.3791/63695-v
Chapters
0:03
Introduction
2:20
Culture Preparation of Salmonella Typhimurium
5:15
Mice and Infections
5:56
CFU Assessment of Organs
9:06
Flow Cytometric Analysis of Various Immune Cell Populations in Peritoneal Exudate
11:56
Results
13:22
Conclusion
Transcript
脓毒症是对微生物侵袭或组织损伤的宿主免疫反应失调,导致器官损伤在远离感染或损伤的部位。在COVID-19大流行期间,公众对这种疾病的认识越来越高。2017年,全世界有4890万例脓毒症发病率和1100万例死亡,占全球死亡总数的近20%。
可以预料,大多数病例都在医院。事实上,一项研究发现,ICU脓毒症患者的阳性分离株中几乎有62%是革兰氏阴性菌。有几种模型,一些常用的脓毒症小鼠模型包括LPS诱导的内毒素血症,盲肠结扎和穿刺模型,以及单细菌感染模型系统。
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Summary
该协议描述了小鼠模型系统中革兰氏阴性单细菌性脓毒症的诱导。该模型有助于研究脓毒症期间的炎症和致死宿主反应。
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