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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

DOI :

10.3791/63709-v

9:57 min

July 25th, 2022

July 25th, 2022

3,016 Views

1Dipartimento di Fisica, Politecnico di Milano, 2Institute for Photonics and Nanotechnologies (IFN-CNR)

We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

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Multiplex Chemical Imaging

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