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JoVE Journal

Neuroscience

This content is Free Access.

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

3.4K Views

05:40 min

May 5th, 2022

May 5th, 2022

3,387 Views

0:04

Introduction

0:49

Cortical Brain Organoids Generation

2:33

Characterization of Neuronal Aging in Cortical Organoids

3:20

Results: Neuroectodermal Differentiation and Characterization of Cortical Brain Organoids and Their Senescence

4:59

Conclusion

Transcript

In this study, we provide a detailed technique for a simple yet robust cortical organoid culture system using standard feeder-free hPSC cultures. This is a rapid, efficient, and reproducible protocol for generating organoids that model aspects of brain senescence in vitro.

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