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DOI :
10.3791/63720-v
April 11th, 2022
Chapters
0:09
Introduction
1:05
Concentration Determination of RNA Solution
3:15
Hydrolysis of Oligonucleotides of RNA by Xrn-1
5:31
Desalting of RNA Solution, MALDI-TOF Plate Spotting
7:41
Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry
8:32
Conclusion
MALDI−TOFを用いて、酸化されたRNAとエキソリボヌクレアーゼXrn−1との間の反応性から得られた断片を特徴付けた。本プロトコルは、RNAおよび/またはDNAを含む他のプロセスに適用できる方法論を記載する。
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