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05:27 min
July 20th, 2022
DOI :
10.3791/63729-v
Chapters
0:04
Introduction
0:40
Measuring the Activity of Complex I + Complex III
2:39
Measuring the Activity of Complex II + Complex III
4:04
Results: Cytochrome c Reduction by Mouse Liver Mitochondrial Membranes
4:54
Conclusion
Transcript
该协议突出了钠作为第二信使的作用,并且还显示了部分分化的泛醇池的存在。该技术展示了一种非常简单的泛醇池研究方法,否则需要非常特定的细胞模型。这种方法可用于研究其他膜中的电子转移,特别是脂质液滴中的酶。
将样品分成四个子样品,每个子样品20微克,并将它们标记为A到D.将样品A和B分成两个子样品,每个子样品10微克,并将它们标记为A1,A2,B1和B2。按照文本协议中所述准备C1 / C2缓冲液,并预热至37摄氏度。在一毫升比色皿中,将每个亚样品加入30微升细胞色素c和10微升丙二
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Summary
该协议描述了一种比较测定,在存在或不存在Na +的情况下使用线粒体复合物活性CI + CIII和CII + CIII,以研究部分分段功能CoQ池的存在。
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