June 8th, 2022
•The present protocol describes the study of neutrophil-biofilm interactions. Staphylococcus aureus biofilms are established in vitro and incubated with peripheral blood-derived human neutrophils. The oxidative burst response from neutrophils is quantified, and the neutrophil localization within the biofilm is determined by microscopy.
Tags
Related Videos
Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus (MRSA)
Experimental Endocarditis Model of Methicillin Resistant Staphylococcus aureus (MRSA) in Rat
Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved